Hotspot

ycf3

LSC · 43281-45244 · 1964 bp

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Pi 0.000933

MarkerSeek score 49.00

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1964
variable_sites: 3
pi: 0.000933
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGATTGATGGGGCCAATAAACA	AGTGGGATCATTTTCTTGCGA	40.9	42.9	58.3	57.9	2141-2144 bp	1.0	53.3
ycf3_p2	CTGATTGATGGGGCCAATAAAC	AGTGGGATCATTTTCTTGCGA	45.5	42.9	57.7	57.9	2142-2145 bp	1.0	52.9
ycf3_p3	GATTGATGGGGCCAATAAACA	AGTGGGATCATTTTCTTGCGA	42.9	42.9	56.5	57.9	2140-2143 bp	1.0	51.8
ycf3_p4	TGATTGATGGGGCCAATAAAC	AGTGGGATCATTTTCTTGCGA	42.9	42.9	56.5	57.9	2141-2144 bp	1.0	51.8
ycf3_p5	TGGGGCCAATAAACAATTCA	AGTGGGATCATTTTCTTGCGA	40.0	42.9	55.5	57.9	2134-2137 bp	1.0	51.3