Hotspot

ycf1

SSC · 124651-130281 · 5631 bp

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Pi 0.00195

MarkerSeek score 44.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5631
variable_sites: 17
pi: 0.00195
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAACGGATCCAAGATTCCCC	CCGTGGGTTATACCAAGCAA	55.0	50.0	58.0	57.9	223 bp	1.0	79.9
ycf1_p2	GAACGGATCCAAGATTCCCC	AACCGTGGGTTATACCAAGC	55.0	50.0	58.0	57.9	225 bp	1.0	79.9
ycf1_p3	CTTTGTCCTTGTTGGGGGAA	CCGTGGGTTATACCAAGCAA	50.0	50.0	57.9	57.9	240 bp	1.0	79.4
ycf1_p4	CTTTGTCCTTGTTGGGGGAA	AACCGTGGGTTATACCAAGC	50.0	50.0	57.9	57.9	242 bp	1.0	79.4
ycf1_p5	TTTGTCCTTGTTGGGGGAAC	CCGTGGGTTATACCAAGCAA	50.0	50.0	58.2	57.9	239 bp	1.0	78.3