Hotspot

rpoA

LSC · 79008-80021 · 1014 bp

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Pi 0.00197

MarkerSeek score 26.60

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1014
variable_sites: 3
pi: 0.00197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TCTTACTCGAGCCGGATGAT	TAGTTCACGTAAGAGTGCGC	50.0	50.0	58.0	58.0	2190-2191 bp	1.0	79.9
rpoA_p2	AAAACCCATTTCGTCGTCCA	TAGTTCACGTAAGAGTGCGC	45.0	50.0	58.0	58.0	1756-1757 bp	1.0	79.8
rpoA_p3	TCTTACTCGAGCCGGATGAT	CTGTTACAGATGTACGGGGC	50.0	55.0	58.0	58.1	2113-2114 bp	1.0	79.7
rpoA_p4	CTTACTCGAGCCGGATGATG	TAGTTCACGTAAGAGTGCGC	55.0	50.0	57.9	58.0	2189-2190 bp	1.0	79.6
rpoA_p5	CTCGAGCCGGATGATGAAAA	TAGTTCACGTAAGAGTGCGC	50.0	50.0	58.1	58.0	2185-2186 bp	1.0	79.6