Hotspot

rbcL

LSC · 55945-57387 · 1443 bp

Back to Gardenia

Pi 0.00219

MarkerSeek score 50.80

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1443
variable_sites: 5
pi: 0.00219
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCATGAGTTGTAGGGAGGGA	AGTAAAAGATTGGGCCGAGT	50.0	45.0	57.1	56.8	1501 bp	1.0	63.4
rbcL_p2	TTCATGAGTTGTAGGGAGGGA	AGTAAAAGATTGGGCCGAGT	47.6	45.0	57.8	56.8	1502 bp	1.0	61.3
rbcL_p3	TCATGAGTTGTAGGGAGGGA	AGTAAAAGATTGGGCCGAGTT	50.0	42.9	57.1	57.6	1501 bp	1.0	61.3
rbcL_p4	TCATGAGTTGTAGGGAGGGAT	AGTAAAAGATTGGGCCGAGT	47.6	45.0	57.6	56.8	1501 bp	1.0	59.5
rbcL_p5	TTCATGAGTTGTAGGGAGGGA	AGTAAAAGATTGGGCCGAGTT	47.6	42.9	57.8	57.6	1502 bp	1.0	59.2