Hotspot

ndhF-rpl32

SSC · 113274-114080 · 807 bp

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Pi 0.0031

MarkerSeek score 47.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 807
variable_sites: 4
pi: 0.0031
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	GGCTGTCCAATATCCCTTCC	50.0	55.0	58.0	57.7	989-1019 bp	1.0	77.8
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	GGCTGTCCAATATCCCTTCC	45.0	55.0	58.0	57.7	987-1017 bp	1.0	77.7
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	GGCTGTCCAATATCCCTTCC	45.0	55.0	58.0	57.7	988-1018 bp	1.0	77.7
ndhF-rpl32_p4	GGGATGATCCACCCGTATTG	GGCTGTCCAATATCCCTTCC	55.0	55.0	57.8	57.7	926-956 bp	1.0	76.6
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	GGCTGTCCAATATCCCTTCC	45.0	55.0	57.8	57.7	992-1022 bp	1.0	76.5