Hotspot

accD-psaI

LSC · 59554-60295 · 742 bp

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Pi 0.00517

MarkerSeek score 45.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 6
pi: 0.00517
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ATTTCAGTTCCACGCTTTCG	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.0	58.0	850-864 bp	1.0	74.2
accD-psaI_p2	ATTTCAGTTCCACGCTTTCG	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.0	57.9	856-870 bp	1.0	73.5
accD-psaI_p3	ATTTCAGTTCCACGCTTTCG	GCCGGAAATACTAGGCCTAC	45.0	55.0	57.0	57.5	839-853 bp	1.0	70.8
accD-psaI_p4	CGCTTTCGTTCCTTTGAATCA	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.5	58.0	838-852 bp	1.0	69.7
accD-psaI_p5	CGCTTTCGTTCCTTTGAATCA	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.5	57.9	844-858 bp	1.0	69.0