Hotspot

rbcL-accD

LSC · 57388-58005 · 618 bp

Back to Gardenia

Pi 0.00324

MarkerSeek score 38.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 618
variable_sites: 3
pi: 0.00324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	TTTATCCCACACCGGCATTC	50.0	50.0	57.9	58.2	809-810 bp	1.0	77.4
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	CGGCATTCGAACTCCTTCTT	50.0	50.0	57.9	58.3	797-798 bp	1.0	77.2
rbcL-accD_p3	CGCGAGGCTAGTAAATGGAG	TTTATCCCACACCGGCATTC	55.0	50.0	58.2	58.2	842-843 bp	1.0	76.5
rbcL-accD_p4	CGATTCAATTTTGACGCGGT	TTTATCCCACACCGGCATTC	45.0	50.0	57.8	58.2	782-783 bp	1.0	76.4
rbcL-accD_p5	CGCGAGGCTAGTAAATGGAG	CGGCATTCGAACTCCTTCTT	55.0	50.0	58.2	58.3	830-831 bp	1.0	76.3