Hotspot

trnT-UGU-trnL-UAA

LSC · 47117-47880 · 764 bp

Back to Gardenia

Pi 0.0024

MarkerSeek score 41.70

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 3
pi: 0.0024
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GGGTTTCCATACCAAGGCTC	55.0	55.0	57.9	58.2	858-860 bp	1.0	77.3
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	825-827 bp	1.0	77.2
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	GGGTTTCCATACCAAGGCTC	50.0	55.0	57.8	58.2	890-892 bp	1.0	76.5
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	857-859 bp	1.0	76.4
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	819-821 bp	1.0	75.9