Hotspot

trnG-GCC-trnfM-CAU

LSC · 37195-37382 · 188 bp

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Pi 0.00621
MarkerSeek score 47.20
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
188
variable_sites
2
pi
0.00621
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 290 bp 1.0 79.9
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 291 bp 1.0 79.9
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 254 bp 1.0 79.9
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 255 bp 1.0 79.9
trnG-GCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC GTCACGGGTTCAAATCCTGT 50.0 50.0 58.1 58.1 248 bp 1.0 79.3