Hotspot

petN-psbM

LSC · 28829-29986 · 1158 bp

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Pi 0.00432

MarkerSeek score 51.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1158
variable_sites: 9
pi: 0.00432
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACGGCCTTTCTACT	50.0	47.6	58.2	58.2	1299 bp	1.0	69.9
petN-psbM_p2	GGGGAAGGAGTGGACTCTAA	AGTTCCTACGGCCTTTCTACT	55.0	47.6	57.8	58.2	1236 bp	1.0	69.1
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACGGCCTTTCTAC	50.0	50.0	58.2	56.7	1299 bp	1.0	68.6
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	GTTCCTACGGCCTTTCTACT	50.0	50.0	58.2	56.7	1298 bp	1.0	68.6
petN-psbM_p5	GGGGGCATAATTCACATGGA	AGTTCCTACGGCCTTTCTACT	50.0	47.6	57.6	58.2	1321 bp	1.0	68.0