Hotspot

trnQ-UUG-psbk

LSC · 7695-8032 · 338 bp

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Pi 0.00542

MarkerSeek score 47.50

Primer available yes

Label trnQ-UUG-psbk

Feature Metadata

label_name: trnQ-UUG-psbk
feature_type: igs
region: LSC
length_bp: 338
variable_sites: 3
pi: 0.00542
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbk_p1	GATATGCTCTGGGACGGAAG	AAAAAGCATAGGCCTCGGG	55.0	52.6	57.6	57.8	515 bp	1.0	67.4
trnQ-UUG-psbk_p2	GACCAAAACCCGTTGCCTTA	AAAAAGCATAGGCCTCGGG	50.0	52.6	58.7	57.8	473 bp	1.0	65.5
trnQ-UUG-psbk_p3	AATGGATATGCTCTGGGACG	AAAAAGCATAGGCCTCGGG	50.0	52.6	57.2	57.8	519 bp	1.0	64.2
trnQ-UUG-psbk_p4	GAATAGCGGGACCAAAACCC	AAAAAGCATAGGCCTCGGG	55.0	52.6	58.9	57.8	482 bp	1.0	63.8
trnQ-UUG-psbk_p5	ATATGCTCTGGGACGGAAGG	AAAAAGCATAGGCCTCGGG	55.0	52.6	58.9	57.8	514 bp	1.0	63.4