Hotspot

rps16-trnQ-UUG

LSC · 6165-7622 · 1458 bp

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Pi 0.00514

MarkerSeek score 43.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1458
variable_sites: 12
pi: 0.00514
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1531-1532 bp	1.0	78.9
rps16-trnQ-UUG_p2	GGATCGTCTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	57.8	58.1	1545-1546 bp	1.0	78.4
rps16-trnQ-UUG_p3	GATCGTCTCCTTCAAGTCGC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1544-1545 bp	1.0	76.4
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1535-1536 bp	1.0	74.2
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1561-1562 bp	1.0	74.1