Hotspot

ccsA-ndhD

SSC · 116695-116998 · 304 bp

Back to Garcinia

Pi 0.0416

MarkerSeek score 70.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 304
variable_sites: 37
pi: 0.0416
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCCGCAATTGTCGCTTCTA	CGAAATGGGTGGAATGGCTA	45.0	50.0	57.9	57.9	820-839 bp	1.0	87.8
ccsA-ndhD_p2	CCAATGAAGCATGGGGATCA	CGAAATGGGTGGAATGGCTA	50.0	50.0	57.9	57.9	935-954 bp	1.0	87.3
ccsA-ndhD_p3	CCAATGAAGCATGGGGATCA	CAACATCTTCTGGTCAGCGA	50.0	50.0	57.9	57.9	1143-1162 bp	0.947368	83.7
ccsA-ndhD_p4	CCAATGAAGCATGGGGATCA	TCAACATCTTCTGGTCAGCG	50.0	50.0	57.9	57.9	1144-1163 bp	0.947368	83.7
ccsA-ndhD_p5	ATTCCGTTGTTCCTTCTCGG	CGAAATGGGTGGAATGGCTA	50.0	50.0	57.9	57.9	1064-1083 bp	0.894737	82.0