Hotspot

psbE-petL

LSC · 65709-66682 · 974 bp

Back to Garcinia

Pi 0.0257

MarkerSeek score 67.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 974
variable_sites: 89
pi: 0.0257
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAGGATCAAAACGGCCAGTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1752-1796 bp	1.0	88.5
psbE-petL_p2	AAAGGATCAAAACGGCCAGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1753-1797 bp	1.0	88.4
psbE-petL_p3	AAAGGATCAAAACGGCCAGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1731-1775 bp	1.0	88.3
psbE-petL_p4	AAGGATCAAAACGGCCAGTT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1730-1774 bp	1.0	88.3
psbE-petL_p5	GAAACGGTAGGTACAGCCAG	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.0	58.0	1873-1917 bp	1.0	88.2