Hotspot

atpB-rbcL

LSC · 54950-55701 · 752 bp

Back to Garcinia

Pi 0.0339

MarkerSeek score 72.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 78
pi: 0.0339
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ACGCCCCAGATTCTTTGTTT	CGGTCTCTCCAACGCATAAA	45.0	50.0	58.0	58.0	1237-1485 bp	1.0	89.0
atpB-rbcL_p2	CTTTTCCTCCACGGCGATAA	CGGTCTCTCCAACGCATAAA	50.0	50.0	58.0	58.0	1673-1921 bp	1.0	88.3
atpB-rbcL_p3	TTTTCCTCCACGGCGATAAG	CGGTCTCTCCAACGCATAAA	50.0	50.0	58.0	58.0	1672-1920 bp	1.0	88.3
atpB-rbcL_p4	TCAACAGGTTCTCCAAGCAC	CGGTCTCTCCAACGCATAAA	50.0	50.0	58.0	58.0	1527-1775 bp	1.0	88.3
atpB-rbcL_p5	AATTCGTCCTAAAGTCGCCC	CGGTCTCTCCAACGCATAAA	50.0	50.0	58.0	58.0	1501-1749 bp	0.526316	68.4