Hotspot

trnT-GGU-psbD

LSC · 32870-33919 · 1050 bp

Back to Garcinia

Pi 0.019

MarkerSeek score 64.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1050
variable_sites: 89
pi: 0.019
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	TCTCCGTAACCAGTCATCCA	50.0	50.0	58.3	57.8	1117-1357 bp	1.0	85.7
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	GGTCTCTCCGTAACCAGTCA	50.0	55.0	58.3	58.5	1121-1361 bp	1.0	83.8
trnT-GGU-psbD_p3	GCCATGGTAAGGCGTAAGTC	TCTCCGTAACCAGTCATCCA	55.0	50.0	58.7	57.8	1127-1367 bp	1.0	82.3
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	CTCTCCGTAACCAGTCATCC	50.0	55.0	58.3	57.1	1118-1358 bp	1.0	80.5
trnT-GGU-psbD_p5	GCCATGGTAAGGCGTAAGTC	GGTCTCTCCGTAACCAGTCA	55.0	55.0	58.7	58.5	1131-1371 bp	1.0	80.4