Hotspot

trnH-GUG-psbA

LSC · 69-579 · 511 bp

Back to Garcinia

Pi 0.0639
MarkerSeek score 82.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
511
variable_sites
102
pi
0.0639
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.90
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 385-580 bp 0.947368 88.3
trnH-GUG-psbA_p2 GCCTTGATCCACTTGGCTAC TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.6 57.9 390-585 bp 0.947368 84.4
trnH-GUG-psbA_p3 CCTTGATCCACTTGGCTACA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.2 57.9 389-584 bp 0.947368 83.0
trnH-GUG-psbA_p4 GCGCATGATGGATTCACAAT TTCCCTCTAGACCTAGCTGC 45.0 55.0 57.2 57.9 415-610 bp 0.947368 83.0
trnH-GUG-psbA_p5 ATTGCCTTGATCCACTTGGC TTCCCTCTAGACCTAGCTGC 50.0 55.0 58.8 57.9 393-588 bp 0.947368 82.8