Hotspot

rpl16

LSC · 80684-82061 · 1378 bp

Back to Gamochaeta

Pi 0.00437

MarkerSeek score 61.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1378
variable_sites: 6
pi: 0.00437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCACCAAAAACTCGAGTTC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2433 bp	1.0	79.8
rpl16_p2	ACACATCAATTCTCGAGCCC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2193 bp	1.0	79.8
rpl16_p3	TCGACGATTGCTAGCTCCTA	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2223 bp	1.0	79.7
rpl16_p4	GCGTCTTTGATCACAGCAAC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2267 bp	1.0	79.6
rpl16_p5	CGCACCAAAAACTCGAGTTC	GCAAATTGCAGGACGTATCG	50.0	50.0	58.0	57.9	2116 bp	1.0	79.6