Hotspot

rbcL

LSC · 54912-56369 · 1458 bp

Back to Gamochaeta

Pi 0.00549

MarkerSeek score 61.90

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1458
variable_sites: 8
pi: 0.00549
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTCATTGAAA	50.0	45.0	57.1	58.0	1521 bp	1.0	72.8
rbcL_p2	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTCATTGAA	50.0	45.0	57.1	58.0	1522 bp	1.0	72.8
rbcL_p3	ACGCCTAATTCATGTCGAGT	TGGGCCGAGTTTCATTGAAA	45.0	45.0	57.0	58.0	1569 bp	1.0	72.6
rbcL_p4	ACGCCTAATTCATGTCGAGT	TTGGGCCGAGTTTCATTGAA	45.0	45.0	57.0	58.0	1570 bp	1.0	72.6
rbcL_p5	TCATGAGTTGTAGGGAGGGA	ATTGGGCCGAGTTTCATTGA	50.0	45.0	57.1	57.8	1523 bp	1.0	71.2