Hotspot

ndhD

SSC · 119605-121134 · 1530 bp

Back to Gamochaeta

Pi 0.00196

MarkerSeek score 46.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 3
pi: 0.00196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ATTTTTGGGCACAGGTTTTT	TCATTAGCCAACCGACTCTT	35.0	45.0	54.7	56.6	1603 bp	1.0	42.2
ndhD_p2	ATTTTTGGGCACAGGTTTTT	TTCATTAGCCAACCGACTCT	35.0	45.0	54.7	56.6	1604 bp	1.0	42.2
ndhD_p3	ATTTTTGGGCACAGGTTTTT	TTCATTAGCCAACCGACTCTT	35.0	42.9	54.7	57.3	1604 bp	1.0	40.2
ndhD_p4	ATTTTTGGGCACAGGTTTTT	TTTCATTAGCCAACCGACTCT	35.0	42.9	54.7	57.3	1605 bp	1.0	40.2
ndhD_p5	ATTTTTGGGCACAGGTTTTT	TTTCATTAGCCAACCGACTCTT	35.0	40.9	54.7	58.0	1605 bp	1.0	40.1