Hotspot

ndhD-ccsA

SSC · 121135-121399 · 265 bp

Back to Gamochaeta

Pi 0.034

MarkerSeek score 72.00

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 265
variable_sites: 9
pi: 0.034
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TCAGTTGACAAGGTTGAAGGT	GCGACTCTCGGCTTTCTTAT	42.9	50.0	57.4	57.8	415 bp	1.0	66.9
ndhD-ccsA_p2	ATCAGTTGACAAGGTTGAAGGT	GCGACTCTCGGCTTTCTTAT	40.9	50.0	57.8	57.8	416 bp	1.0	62.7
ndhD-ccsA_p3	CAGTTGACAAGGTTGAAGGT	GCGACTCTCGGCTTTCTTAT	45.0	50.0	55.8	57.8	414 bp	1.0	62.4
ndhD-ccsA_p4	TCAGTTGACAAGGTTGAAGG	GCGACTCTCGGCTTTCTTAT	45.0	50.0	55.5	57.8	415 bp	1.0	60.1
ndhD-ccsA_p5	TCAGTTGACAAGGTTGAAGGTA	GCGACTCTCGGCTTTCTTAT	40.9	50.0	57.2	57.8	415 bp	1.0	57.9