Hotspot

psaC-ndhD

SSC · 119515-119604 · 90 bp

Back to Gamochaeta

Pi 0.0333
MarkerSeek score 71.70
Primer available yes
Label psaC-ndhD

Feature Metadata

label_name
psaC-ndhD
feature_type
igs
region
SSC
length_bp
90
variable_sites
3
pi
0.0333
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhD_p1 ACAGAGGATTGTGTCGGTTG AGGAACCCGCAAATATTGGT 50.0 45.0 57.8 57.5 289 bp 1.0 75.3
psaC-ndhD_p2 CAGAGGATTGTGTCGGTTGT AGGAACCCGCAAATATTGGT 50.0 45.0 57.8 57.5 288 bp 1.0 75.3
psaC-ndhD_p3 TTATGGCATGAAACAACGCG AGGAACCCGCAAATATTGGT 45.0 45.0 57.7 57.5 217 bp 1.0 74.9
psaC-ndhD_p4 AAGAGATGTGAATCCGCCTG AGGAACCCGCAAATATTGGT 50.0 45.0 57.7 57.5 268 bp 1.0 74.1
psaC-ndhD_p5 CGCAGCATGGGTATAGCTTA AGGAACCCGCAAATATTGGT 50.0 45.0 57.5 57.5 199 bp 1.0 73.4