Hotspot

trnI-GAU-trnA-UGC

IRb · 102187-102250 · 64 bp

Back to Gamochaeta

Pi 0.0156

MarkerSeek score 61.50

Primer available yes

Label trnI-GAU-trnA-UGC

Feature Metadata

label_name: trnI-GAU-trnA-UGC
feature_type: igs
region: IRb
length_bp: 64
variable_sites: 1
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-trnA-UGC_p1	TGATTTACTTCACGGGCGAG	CAACGACCCAATTGCAAGAG	50.0	50.0	58.0	57.7	161 bp	1.0	77.8
trnI-GAU-trnA-UGC_p2	TGATTTACTTCACGGGCGAG	CCGCCTGGACAATTAGACAT	50.0	50.0	58.0	57.7	195 bp	1.0	77.7
trnI-GAU-trnA-UGC_p3	AGGTCTCTGGTTCAAGTCCA	CCGCCTGGACAATTAGACAT	50.0	50.0	57.9	57.7	177 bp	1.0	77.0
trnI-GAU-trnA-UGC_p4	AGGTCTCTGGTTCAAGTCCA	CAACGACCCAATTGCAAGAG	50.0	50.0	57.9	57.7	143 bp	1.0	77.0
trnI-GAU-trnA-UGC_p5	TGATTTACTTCACGGGCGAG	AGACATCCAACCCGTAATCG	50.0	50.0	58.0	57.4	181 bp	1.0	75.6