Hotspot

accD-psaI

LSC · 58375-59171 · 797 bp

Back to Gamochaeta

Pi 0.00502

MarkerSeek score 61.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 4
pi: 0.00502
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	TTGCGATTGCCGGAAATACT	47.6	45.0	57.2	58.3	968-984 bp	1.0	64.3
accD-psaI_p2	CCAGAAGGGCTTATTCGATCT	TTGCGATTGCCGGAAATACT	47.6	45.0	57.2	58.3	967-983 bp	1.0	64.2
accD-psaI_p3	TTCCAGAAGGGCTTATTCGA	TTGCGATTGCCGGAAATACT	45.0	45.0	56.2	58.3	969-985 bp	1.0	64.2
accD-psaI_p4	TCCAGAAGGGCTTATTCGATC	AGGCCTACTAAAGGGACCAA	47.6	50.0	57.2	57.6	948-964 bp	1.0	63.5
accD-psaI_p5	CCAGAAGGGCTTATTCGATCT	AGGCCTACTAAAGGGACCAA	47.6	50.0	57.2	57.6	947-963 bp	1.0	63.4