Hotspot

trnF-GAA-ndhJ

LSC · 47741-48314 · 574 bp

Back to Gamochaeta

Pi 0.0089

MarkerSeek score 65.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 574
variable_sites: 5
pi: 0.0089
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	668-677 bp	1.0	71.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	667-676 bp	1.0	71.8
trnF-GAA-ndhJ_p3	TAGTCGGGATAGCTCAGTCG	TGGATAGGATGGCCTTTACG	55.0	50.0	58.1	56.8	709-718 bp	1.0	71.2
trnF-GAA-ndhJ_p4	TAGTCGGGATAGCTCAGTCG	GGATAGGATGGCCTTTACGT	55.0	50.0	58.1	56.8	708-717 bp	1.0	71.2
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	50.0	47.6	58.1	57.7	675-684 bp	1.0	71.2