Hotspot

psbZ-trnG-GCC

LSC · 35429-35731 · 303 bp

Back to Gamochaeta

Pi 0.0429

MarkerSeek score 69.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 303
variable_sites: 13
pi: 0.0429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	ACTTCATTTTGGGCGGGTAG	50.0	50.0	57.8	58.2	495 bp	1.0	79.1
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GACTTCATTTTGGGCGGGTA	50.0	50.0	57.8	58.2	496 bp	1.0	79.1
psbZ-trnG-GCC_p3	GCTTCTCCTGATGGTTGGTC	ACTTCATTTTGGGCGGGTAG	55.0	50.0	58.3	58.2	493 bp	1.0	78.7
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	GACTTCATTTTGGGCGGGTA	55.0	50.0	58.3	58.2	494 bp	1.0	78.7
psbZ-trnG-GCC_p5	TTGCTTCTCCTGATGGTTGG	ATGACTTCATTTTGGGCGGG	50.0	50.0	57.8	58.8	498 bp	1.0	73.9