Hotspot

trnS-GCU-trnC-GCA

LSC · 8635-9357 · 723 bp

Back to Gamochaeta

Pi 0.00714
MarkerSeek score 54.70
Primer available yes
Label trnS-GCU-trnC-GCA

Feature Metadata

label_name
trnS-GCU-trnC-GCA
feature_type
igs
region
LSC
length_bp
723
variable_sites
5
pi
0.00714
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnC-GCA_p1 CTTTCGTCCACTCAGCCATC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.6 58.1 787-810 bp 1.0 77.1
trnS-GCU-trnC-GCA_p2 CTTTCGTCCACTCAGCCATC AAAAGGATTTGCAGTCCCCC 55.0 50.0 58.6 58.4 769-792 bp 1.0 74.9
trnS-GCU-trnC-GCA_p3 TTTCGTCCACTCAGCCATCT CCGGATTTGAACTGGGGAAA 50.0 50.0 59.0 58.1 786-809 bp 1.0 74.0
trnS-GCU-trnC-GCA_p4 TCCACTCAGCCATCTCTCC CCGGATTTGAACTGGGGAAA 57.9 50.0 58.1 58.1 781-804 bp 1.0 73.4
trnS-GCU-trnC-GCA_p5 ATTAGCAATCCGCCGCTTT CCGGATTTGAACTGGGGAAA 47.4 50.0 58.2 58.1 802-825 bp 1.0 72.4