Hotspot

psbL

LSC · 64375-64491 · 117 bp

Back to Gamocarpha

Pi 0.0171

MarkerSeek score 62.30

Primer available yes

Label psbL

Feature Metadata

label_name: psbL
feature_type: gene
region: LSC
length_bp: 117
variable_sites: 2
pi: 0.0171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL_p1	ATGAGATGAGAGCCTTCCGA	TCAGCAATGCAGTTCATCCA	50.0	45.0	57.9	57.8	243 bp	1.0	78.4
psbL_p2	GATGAGAGCCTTCCGAATGG	TCAGCAATGCAGTTCATCCA	55.0	45.0	58.1	57.8	239 bp	1.0	78.1
psbL_p3	TGAGATGAGAGCCTTCCGAA	TCAGCAATGCAGTTCATCCA	50.0	45.0	58.1	57.8	242 bp	1.0	77.9
psbL_p4	CCTTCCGAATGGGCTAAGAG	TCAGCAATGCAGTTCATCCA	55.0	45.0	57.8	57.8	231 bp	1.0	77.1
psbL_p5	ATGAGATGAGAGCCTTCCGA	GCAATGCAGTTCATCCAACG	50.0	50.0	57.9	58.4	240 bp	1.0	77.0