Hotspot

rpl32-trnL-UAG

SSC · 112040-112911 · 872 bp

Back to Gamocarpha

Pi 0.00583
MarkerSeek score 63.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
872
variable_sites
5
pi
0.00583
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCGTTAAAAGCTTTGT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 989-993 bp 1.0 80.4
rpl32-trnL-UAG_p2 GGCAGCGTTAAAAGCTTTGT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 990-994 bp 1.0 80.4
rpl32-trnL-UAG_p3 AAAGGATATTGGGCAGCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 1000-1004 bp 1.0 80.3
rpl32-trnL-UAG_p4 AAAGGATATTGGGCAGCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 1001-1005 bp 1.0 80.3
rpl32-trnL-UAG_p5 GGCAGCGTTAAAAGCTTTGT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 999-1003 bp 1.0 79.5