Hotspot

ndhF-rpl32

SSC · 111194-111874 · 681 bp

Back to Gamocarpha

Pi 0.00587

MarkerSeek score 59.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 681
variable_sites: 4
pi: 0.00587
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	ACAAAGCTTTTAACGCTGCC	50.0	45.0	58.0	57.9	876-1124 bp	1.0	79.0
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	AACGCTGCCCAATATCCTTT	50.0	45.0	58.0	57.9	865-1113 bp	1.0	78.9
ndhF-rpl32_p3	TGCCGTCGGAAAAAGAAGAA	ACAAAGCTTTTAACGCTGCC	45.0	45.0	58.0	57.9	874-1122 bp	1.0	78.9
ndhF-rpl32_p4	TTGCCGTCGGAAAAAGAAGA	ACAAAGCTTTTAACGCTGCC	45.0	45.0	58.0	57.9	875-1123 bp	1.0	78.9
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	AACGCTGCCCAATATCCTTT	45.0	45.0	58.0	57.9	863-1111 bp	1.0	78.8