Hotspot

rps19-rpl2

IRb · 83903-83955 · 53 bp

Back to Gamocarpha

Pi 0.0377

MarkerSeek score 74.20

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 53
variable_sites: 2
pi: 0.0377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCCCGGGACCAGGTTATTAT	GGGCCCCAATTGGTAGAAAA	55.0	50.0	59.2	58.1	282-283 bp	1.0	70.3
rps19-rpl2_p2	GCCCGGGACCAGGTTATTAT	TTGATTCTTCGTCGCCGTAG	55.0	50.0	59.2	58.1	190-191 bp	1.0	70.2
rps19-rpl2_p3	GCCCGGGACCAGGTTATTAT	CACAACCCCTTGGGGTTATC	55.0	55.0	59.2	58.2	257-258 bp	1.0	69.6
rps19-rpl2_p4	CCCGGGACCAGGTTATTATT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	56.7	58.1	281-282 bp	1.0	69.4
rps19-rpl2_p5	CCCGGGACCAGGTTATTATT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	56.7	58.1	189-190 bp	1.0	69.4