Hotspot

pafII-cemA

LSC · 60641-61505 · 865 bp

Back to Gamocarpha

Pi 0.00694

MarkerSeek score 62.90

Primer available yes

Label pafII-cemA

Feature Metadata

label_name: pafII-cemA
feature_type: igs
region: LSC
length_bp: 865
variable_sites: 6
pi: 0.00694
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafII-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	1044-1045 bp	1.0	79.7
pafII-cemA_p2	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1008-1009 bp	1.0	79.2
pafII-cemA_p3	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	976-977 bp	1.0	79.2
pafII-cemA_p4	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	990-991 bp	1.0	78.6
pafII-cemA_p5	GAGCCATTCCATTGACTCGT	ACCCAGGATTCCAGACCTTT	50.0	50.0	58.0	58.2	1075-1076 bp	1.0	78.4