Hotspot

psaC-ndhE

SSC · 121754-122000 · 247 bp

Back to Gambeya

Pi 0.00607

MarkerSeek score 56.80

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 247
variable_sites: 3
pi: 0.00607
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CTTAGCTTTACACCCGTCCC	TGAAGCAGCTATTGGACTGG	55.0	50.0	58.0	57.6	449 bp	1.0	77.1
psaC-ndhE_p2	TTACACCCGTCCCAAGGTAT	TGAAGCAGCTATTGGACTGG	50.0	50.0	58.0	57.6	442 bp	1.0	76.9
psaC-ndhE_p3	TGCTTAGCTTTACACCCGTC	TGAAGCAGCTATTGGACTGG	50.0	50.0	57.9	57.6	451 bp	1.0	76.6
psaC-ndhE_p4	CTTAGCTTTACACCCGTCCC	AGCAGCTATTGGACTGGCTA	55.0	50.0	58.0	58.5	446 bp	1.0	76.4
psaC-ndhE_p5	TTACACCCGTCCCAAGGTAT	AGCAGCTATTGGACTGGCTA	50.0	50.0	58.0	58.5	439 bp	1.0	76.2