Hotspot

rpl32-trnL-UAG

SSC · 117621-118527 · 907 bp

Back to Gambeya

Pi 0.00753
MarkerSeek score 60.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
907
variable_sites
13
pi
0.00753
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGGCAGCCTTAAAAGCTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.0 57.9 1030-1039 bp 1.0 80.3
rpl32-trnL-UAG_p2 AGGGCAGCCTTAAAAGCTTT AGCAGCGTGTCTACCAATTT 45.0 45.0 58.0 57.8 1023-1032 bp 1.0 79.8
rpl32-trnL-UAG_p3 AGGGCAGCCTTAAAAGCTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.0 57.8 1029-1038 bp 1.0 79.7
rpl32-trnL-UAG_p4 AGGGCAGCCTTAAAAGCTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.0 57.8 1028-1037 bp 1.0 79.7
rpl32-trnL-UAG_p5 AGGGCAGCCTTAAAAGCTTT GAGCAGCGTGTCTACCAATT 45.0 50.0 58.0 58.3 1024-1033 bp 1.0 79.1