Hotspot

ycf2-trnL-CAA

IRb · 97083-97791 · 709 bp

Back to Gambeya

Pi 0.00776

MarkerSeek score 55.80

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 709
variable_sites: 11
pi: 0.00776
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	818 bp	1.0	79.8
ycf2-trnL-CAA_p2	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	817 bp	1.0	79.8
ycf2-trnL-CAA_p3	ATGGCTTTTCCCGGATGAAA	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	820 bp	1.0	79.7
ycf2-trnL-CAA_p4	TGGCTTTTCCCGGATGAAAT	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	819 bp	1.0	79.7
ycf2-trnL-CAA_p5	AAGATGGCTTTTCCCGGATG	AAATGGTAGACACGCGAGAC	50.0	50.0	58.2	58.0	821 bp	1.0	79.7