Hotspot

ycf4-cemA

LSC · 63337-64268 · 932 bp

Back to Gambeya

Pi 0.0034

MarkerSeek score 58.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 932
variable_sites: 6
pi: 0.0034
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	1027-1044 bp	1.0	80.8
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	1041-1058 bp	1.0	80.1
ycf4-cemA_p3	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	1039-1056 bp	1.0	79.8
ycf4-cemA_p4	GCCATTCCCTTGACTCGTAC	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.3	58.1	1093-1110 bp	1.0	78.6
ycf4-cemA_p5	TTGGCCTATTTCTTGCGTGT	AGAAATCCACCAGGGCAAAA	45.0	45.0	58.1	57.6	1026-1043 bp	1.0	78.2