Hotspot

trnG-GCC-trnfM-CAU

LSC · 38819-38988 · 170 bp

Back to Gambeya

Pi 0.0109

MarkerSeek score 58.90

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 170
variable_sites: 3
pi: 0.0109
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TTCTCTTTGCCACGGAGAAG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	249-251 bp	1.0	79.9
trnG-GCC-trnfM-CAU_p2	TTCTCTTTGCCACGGAGAAG	TCAGTTCTTCTCTTCAGCGC	50.0	50.0	57.8	57.9	306-308 bp	1.0	79.4
trnG-GCC-trnfM-CAU_p3	TTCTCTTTGCCACGGAGAAG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.8	58.1	243-245 bp	1.0	79.4
trnG-GCC-trnfM-CAU_p4	TTCTCTTTGCCACGGAGAAG	TCACGGGTTCAAATCCTGTC	50.0	50.0	57.8	57.8	242-244 bp	1.0	78.9
trnG-GCC-trnfM-CAU_p5	TTCTCTTTGCCACGGAGAAG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	247-249 bp	1.0	78.8