Hotspot

petN-psbM

LSC · 29761-30972 · 1212 bp

Back to Gambeya

Pi 0.00798

MarkerSeek score 54.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1212
variable_sites: 17
pi: 0.00798
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTTAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1374-1395 bp	1.0	68.5
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1313-1334 bp	1.0	67.0
petN-psbM_p3	TAGTTAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1374-1395 bp	1.0	64.3
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTC	50.0	45.0	57.6	56.1	1313-1334 bp	1.0	62.8
petN-psbM_p5	ATAGTTAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.1	57.6	1375-1396 bp	1.0	62.6