Hotspot

psbK-psbI

LSC · 7961-8381 · 421 bp

Back to Gambeya

Pi 0.00594

MarkerSeek score 59.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 5
pi: 0.00594
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCTGCCGTAAGTTTTCG	TCTTCACGTCCAGGATTACG	50.0	50.0	58.0	57.1	550-552 bp	1.0	73.2
psbK-psbI_p2	AGCTGCCGTAAGTTTTCGAT	TCTTCACGTCCAGGATTACG	45.0	50.0	57.9	57.1	548-550 bp	1.0	72.8
psbK-psbI_p3	AAGCTGCCGTAAGTTTTCGA	TCTTCACGTCCAGGATTACG	45.0	50.0	58.1	57.1	549-551 bp	1.0	72.5
psbK-psbI_p4	GCTGCCGTAAGTTTTCGATG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.8	57.1	547-549 bp	1.0	72.3
psbK-psbI_p5	GCCAGTCATACCTGTGCTTT	TCTTCACGTCCAGGATTACG	50.0	50.0	58.2	57.1	593-595 bp	1.0	72.1