Hotspot

rps16-trnQ-UUG

LSC · 6460-7354 · 895 bp

Back to Gambeya

Pi 0.00411

MarkerSeek score 63.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 895
variable_sites: 7
pi: 0.00411
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	972-981 bp	1.0	81.1
rps16-trnQ-UUG_p2	TCCTGTCTTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	976-985 bp	1.0	80.0
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	965-974 bp	1.0	80.0
rps16-trnQ-UUG_p4	CCACAACGGATCCTGTCTTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	986-995 bp	1.0	79.6
rps16-trnQ-UUG_p5	GTCTTTCAAGTCGCACGTTG	AAATTTAAATGGGGCGTGGC	50.0	45.0	58.0	57.6	1029-1038 bp	1.0	78.9