Hotspot

ccsA-ndhD

SSC · 114546-114800 · 255 bp

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Pi 0.0527
MarkerSeek score 77.70
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
255
variable_sites
30
pi
0.0527
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CTAGAAAGACGCAGGGTTCC CCGCGCGAGTTATTTCTTTC 55.0 50.0 58.0 57.9 510-530 bp 1.0 88.1
ccsA-ndhD_p2 CTAGAAAGACGCAGGGTTCC TAGACTGCGCCTTGTTTACC 55.0 50.0 58.0 57.9 823-843 bp 1.0 86.0
ccsA-ndhD_p3 CTAGAAAGACGCAGGGTTCC GGGAGCATATGGGTTAGTGC 55.0 55.0 58.0 58.1 1117-1137 bp 1.0 84.7
ccsA-ndhD_p4 CTAGAAAGACGCAGGGTTCC CATATGGGTTAGTGCGGGTC 55.0 55.0 58.0 58.1 1112-1132 bp 1.0 84.6
ccsA-ndhD_p5 CTAGAAAGACGCAGGGTTCC CGATACGGGGCTTAATGGAG 55.0 55.0 58.0 57.9 913-924 bp 0.714286 72.3