Hotspot

rpl32-trnL-UAG

SSC · 112755-113392 · 638 bp

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Pi 0.0568
MarkerSeek score 86.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
638
variable_sites
86
pi
0.0568
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTACAGCAAACAACTAAGT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 52.7 57.9 668-742 bp 1.0 52.0
rpl32-trnL-UAG_p2 TGTACAGCAAACAACTAAGT AGCAGCGTGTCTACCAATTT 35.0 45.0 52.7 57.8 661-735 bp 1.0 52.0
rpl32-trnL-UAG_p3 TGTACAGCAAACAACTAAGT CCTAAGAGCAGCGTGTCTAC 35.0 55.0 52.7 57.8 667-741 bp 1.0 52.0
rpl32-trnL-UAG_p4 TGTACAGCAAACAACTAAGT CTAAGAGCAGCGTGTCTACC 35.0 55.0 52.7 57.8 666-740 bp 1.0 52.0
rpl32-trnL-UAG_p5 TTGTACAGCAAACAACTAAGT TCCTAAGAGCAGCGTGTCTA 33.3 50.0 53.6 57.9 669-743 bp 1.0 52.0