Hotspot

ndhF-rpl32

SSC · 111852-112592 · 741 bp

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Pi 0.0495
MarkerSeek score 84.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
741
variable_sites
89
pi
0.0495
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACACACTCCAACCAATTCCC GAGTTGCCGGTAAAAAGGGA 50.0 50.0 58.0 58.1 1331-1363 bp 0.714286 74.3
ndhF-rpl32_p2 CACACTCCAACCAATTCCCA GAGTTGCCGGTAAAAAGGGA 50.0 50.0 58.0 58.1 1330-1362 bp 0.714286 74.3
ndhF-rpl32_p3 GCAAGAGGTCGTGTAAACCA GAGTTGCCGGTAAAAAGGGA 50.0 50.0 58.1 58.1 1372-1404 bp 0.714286 73.1
ndhF-rpl32_p4 AAGGGGAAATTGTGCCGATT GAGTTGCCGGTAAAAAGGGA 45.0 50.0 58.1 58.1 1598-1630 bp 0.714286 73.0
ndhF-rpl32_p5 AGGGGAAATTGTGCCGATTT GAGTTGCCGGTAAAAAGGGA 45.0 50.0 58.1 58.1 1597-1629 bp 0.714286 73.0