Hotspot

trnL-UAA-trnF-GAA

LSC · 48283-48628 · 346 bp

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Pi 0.0441
MarkerSeek score 80.70
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
346
variable_sites
38
pi
0.0441
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TCTACCAGCTGAGCTATCCC 50.0 55.0 57.5 58.0 400-412 bp 1.0 86.3
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 436-448 bp 1.0 85.0
trnL-UAA-trnF-GAA_p3 AATCGTGAGGGTTCAAGTCC TCAGTCCTCTGCTCTACCAG 50.0 55.0 57.5 57.9 412-424 bp 1.0 84.9
trnL-UAA-trnF-GAA_p4 AATCGTGAGGGTTCAAGTCC CCATGCCAGGAACCAGATTT 50.0 50.0 57.5 58.1 453-465 bp 1.0 84.0
trnL-UAA-trnF-GAA_p5 AATCGTGAGGGTTCAAGTCC GAACTGGTGACACGAGGATT 50.0 50.0 57.5 57.5 433-445 bp 1.0 81.9