Hotspot

psbM-trnD-GUC

LSC · 29710-30284 · 575 bp

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Pi 0.0381
MarkerSeek score 77.90
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
575
variable_sites
49
pi
0.0381
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 ACACAGACATCGTGGTTGTC CATCTCTCTTTCACGGAGGC 50.0 55.0 58.1 58.1 1348-1432 bp 0.857143 81.2
psbM-trnD-GUC_p2 ACACAGACATCGTGGTTGTC TATAACCCCCTTGCCCTTCA 50.0 50.0 58.1 58.0 1130-1214 bp 0.714286 75.5
psbM-trnD-GUC_p3 TACCCCGACCAGAATTAGCT TATAACCCCCTTGCCCTTCA 50.0 50.0 57.8 58.0 922-973 bp 0.714286 75.2
psbM-trnD-GUC_p4 AGACATCGTGGTTGTCGAAC TATAACCCCCTTGCCCTTCA 50.0 50.0 58.2 58.0 1126-1210 bp 0.714286 74.8
psbM-trnD-GUC_p5 CAGACATCGTGGTTGTCGAA TATAACCCCCTTGCCCTTCA 50.0 50.0 58.2 58.0 1127-1211 bp 0.714286 74.8