Hotspot

trnS-GCU-trnG-GCC

LSC · 8610-9194 · 585 bp

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Pi 0.0546
MarkerSeek score 87.30
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
585
variable_sites
71
pi
0.0546
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
87.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 654-857 bp 1.0 63.6
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 653-856 bp 1.0 63.6
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA AACGAATCACACTTTTACCACT 50.0 36.4 58.0 56.2 655-858 bp 1.0 61.7
trnS-GCU-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC AACGAATCACACTTTTACCACT 50.0 36.4 58.0 56.2 654-857 bp 1.0 61.7
trnS-GCU-trnG-GCC_p5 AGCAATCCGACGCTTTAGTC ACGAATCACACTTTTACCACT 50.0 38.1 58.4 55.5 656-859 bp 1.0 60.7