Hotspot

C5T07_pgp085-trnQ-UUG

LSC · 6241-7395 · 1155 bp

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Pi 0.0912
MarkerSeek score 86.50
Primer available yes
Label C5T07_pgp085-trnQ-UUG

Feature Metadata

label_name
C5T07_pgp085-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1155
variable_sites
212
pi
0.0912
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
C5T07_pgp085-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1020-1477 bp 1.0 90.3
C5T07_pgp085-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1024-1481 bp 1.0 85.5
C5T07_pgp085-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1050-1507 bp 1.0 85.4
C5T07_pgp085-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1012-1469 bp 1.0 85.1
C5T07_pgp085-trnQ-UUG_p5 AGTCGCACGTTGCTTTCTAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.9 58.1 1019-1476 bp 1.0 84.5