Hotspot

trnH-GUG-psbA

LSC · 102-335 · 234 bp

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Pi 0.0734
MarkerSeek score 80.30
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
234
variable_sites
40
pi
0.0734
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG AGCTGCTATAGATGCTCCATC 55.0 47.6 58.1 57.1 281-307 bp 1.0 75.8
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA AGCTGCTATAGATGCTCCATC 50.0 47.6 57.9 57.1 295-321 bp 1.0 75.8
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC AGCTGCTATAGATGCTCCATC 50.0 47.6 57.9 57.1 294-320 bp 1.0 75.8
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC AGCTGCTATAGATGCTCCATC 50.0 47.6 57.9 57.1 296-322 bp 1.0 75.8
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG AGCTGCTATAGATGCTCCAT 55.0 45.0 58.1 55.9 281-307 bp 1.0 73.8