Hotspot

C5T07_pgp085

LSC · 4987-6240 · 1254 bp

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Pi 0.0564
MarkerSeek score 84.90
Primer available yes
Label C5T07_pgp085

Feature Metadata

label_name
C5T07_pgp085
feature_type
gene
region
LSC
length_bp
1254
variable_sites
148
pi
0.0564
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
C5T07_pgp085_p1 ACACAAAGCAGAAATGCGTG GCCGTAAGTGTATGTAGCCG 45.0 55.0 57.9 58.5 2235-2410 bp 0.285714 57.1
C5T07_pgp085_p2 CACACAAAGCAGAAATGCGT GCCGTAAGTGTATGTAGCCG 45.0 55.0 57.9 58.5 2236-2411 bp 0.285714 57.1
C5T07_pgp085_p3 CTACCCCCTCCCCCTTTTTA GCCGTAAGTGTATGTAGCCG 55.0 55.0 58.0 58.5 1999 bp 0.142857 41.5
C5T07_pgp085_p4 GGTGCAAAAAGAATCCACCG GCCGTAAGTGTATGTAGCCG 50.0 55.0 57.9 58.5 2378 bp 0.142857 41.1
C5T07_pgp085_p5 ATAAACAGCGATAAGGGGCG GCCGTAAGTGTATGTAGCCG 50.0 55.0 58.1 58.5 2073 bp 0.142857 40.6