Hotspot

ycf1

SSC · 108066-113123 · 5058 bp

Back to Galinsoga

Pi 0.000989

MarkerSeek score 51.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5058
variable_sites: 5
pi: 0.000989
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATGCAAGGGATGGATCACC	GATTTCCTTGAGACGGTCCC	50.0	55.0	57.9	58.0	242 bp	1.0	79.8
ycf1_p2	AATGCAAGGGATGGATCACC	GGATTTCCTTGAGACGGTCC	50.0	55.0	57.9	58.0	243 bp	1.0	79.8
ycf1_p3	AATGCAAGGGATGGATCACC	GGGATTTCCTTGAGACGGTC	50.0	55.0	57.9	58.0	244 bp	1.0	79.8
ycf1_p4	AATGCAAGGGATGGATCACC	GAGACGGTCCCCAAATAACC	50.0	55.0	57.9	58.0	233 bp	1.0	79.8
ycf1_p5	GGATCACCAGCTTCGAACAA	GATTTCCTTGAGACGGTCCC	50.0	55.0	58.2	58.0	230 bp	1.0	79.0